Journal article
Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids
BJ Tauro, DW Greening, RA Mathias, S Mathivanan, H Ji, RJ Simpson
Molecular and Cellular Proteomics | ELSEVIER | Published : 2013
Abstract
Exosomes are naturally occurring biological nanomembranous vesicles (̃40 to 100 nm) of endocytic origin that are released from diverse cell types into the extracellular space. They have pleiotropic functions such as antigen presentation and intercellular transfer of protein cargo, mRNA, microRNA, lipids, and oncogenic potential. Here we describe the isolation, via sequential immunocapture using anti-A33- and anti-EpCAM-coupled magnetic beads, of two distinct populations of exosomes released from organoids derived from human colon carcinoma cell line LIM1863. The exosome populations (A33-Exos and EpCAM-Exos) could not be distinguished via electron microscopy and contained stereotypical exosom..
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Awarded by National Health & Medical Research Council of Australia program
Awarded by National Health & Medical Research Council of Australia
Funding Acknowledgements
This work was supported by the National Health & Medical Research Council of Australia program through Grant No. 487922 (R.J.S.), Fellowship No. 1016599 (S.M.), and Early Career CJ Martin Fellowship No. APP1037043 (R.A.M.). B.J.T. is supported by The University of Melbourne Research Scholarship (MRS). Analysis of proteomic data described in this work was supported using the Australian Proteomics Computational Facility funded by the National Health & Medical Research Council of Australia through Grant No. 381413. This work was supported by funds from the Operational Infrastructure Support Program provided by the Victorian Government Australia. We acknowledge the Australian Cancer Research Foundation for providing funds to purchase the Orbitrap (TM) mass spectrometer.